Construction and confirmation of a recombinant adenovirus vector of survivin.
- Author:
Yuan-Jing LÜ
1
;
Su-Sheng MIAO
;
Wei-Song LIU
;
Hong-Jiang HE
;
Shen-Shan JIA
;
Guo-Qing HE
;
Cheng XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Genetic Vectors; HEK293 Cells; Humans; Inhibitor of Apoptosis Proteins; genetics; metabolism; Plasmids; Polymerase Chain Reaction; Recombinant Fusion Proteins; genetics; metabolism; Transfection
- From: Chinese Journal of Oncology 2011;33(1):13-17
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a recombinant adenovirus of survivin vector and provid valuable reference for gene therapy of laryngeal cancer.
METHODSThe survivin gene was cloned by PCR. After confirmation by enzyme restriction analysis and sequencing, the gene and the adenovirus vector were recombined together to construct the recombinant adenovirus vector. The recombinant adenovirus vector was confirmed via both sequencing and digestion restriction analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenovirus.
RESULTSThe sequence analysis demonstrated that the survivin gene sequence was the same as published in the literature, suggesting that a recombinant adenovirus vector has been successfully constructed.
CONCLUSIONSA survivin recombinant adenovirus has been successfully constructed.
