Expression of LOX and MMP-2 in gastric cancer tissue and the effects of LOX and MMP-2 on tumor invasion and metastasis.
- Author:
Li-Juan MA
1
;
Yi-Gong LI
;
Ling HUANG
;
Mei HAN
;
Bai-Jing MA
;
Bo-Jian SUN
;
Jia-Jing LIN
;
Ting-Ge SONG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; surgery; Biomarkers, Tumor; metabolism; Female; Gastrectomy; Humans; Lymphatic Metastasis; Male; Matrix Metalloproteinase 2; genetics; metabolism; Middle Aged; Neoplasm Invasiveness; Protein-Lysine 6-Oxidase; genetics; metabolism; RNA, Messenger; metabolism; Stomach; metabolism; surgery; Stomach Neoplasms; metabolism; pathology; surgery
- From: Chinese Journal of Oncology 2011;33(1):37-41
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the expressions of lysyl oxidase (LOX) and matrix metalloproteinases-2 (MMP-2) in gastric cancer and pericancerous tissues, in gastric cancers with and without lymph node metastasis, and to analyze the effects of LOX and MMP-2 on tumor invasion and metastasis.
METHODSGastric cancer and pericancerous tissues were collected from 46 patients who underwent surgery. Levels of LOX and MMP-2 mRNA were detected by RT-PCR. Protein abundance of LOX and MMP-2 was examined using Western blot.
RESULTSExpressions of LOX and MMP-2 mRNA, and protein in 46 gastric cancers were significantly higher than that in 46 pericancerous tissues. In gastric cancer with lymph node metastasis, the levels of LOX and MMP-2 mRNA and protein were higher than those in gastric cancers without lymph node metastasis (P < 0.05). In the groups of gastric cancer with lymph node metastasis, expression of LOX was positively correlated with MMP-2 protein expression (P < 0.01).
CONCLUSIONSExpressions of LOX and MMP-2 in gastric cancer tissues are significantly higher than that in pericancerous tissues. The expressions of LOX and MMP-2 in gastric cancer with lymph node metastasis are higher than that in gastric cancer without lymph node metastasis. Expressions of LOX and MMP-2 are positively correlated. The results suggest that LOX and MMP-2 may promote the growth and metastasis of gastric cancer.
