Effects of PTEN gene on invasion and migration of ovarian cancer cell line A2780 and related mechanisms.
- Author:
Hui-juan WU
1
;
Quan HAO
;
Ke WANG
;
Wen-xin LIU
;
Ding MA
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Movement; Down-Regulation; Female; Genetic Vectors; Humans; Matrix Metalloproteinase 9; metabolism; Mutation; Neoplasm Invasiveness; Ovarian Neoplasms; metabolism; pathology; PTEN Phosphohydrolase; genetics; metabolism; physiology; Plasmids; Transfection
- From: Chinese Journal of Oncology 2011;33(3):165-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effects of PTEN on invasive and migration ability of human ovarian cancer cell line A2780 and related mechanisms.
METHODSThe plasmid including WT-PTEN and mutant PTEN were transferred into A2780 cells. The invasive and migration ability were measured before and after transfection by transwell chamber and wound-healing assays. The expression of PTEN protein and related proteins in the cells were detected by Western blot analysis. Empty plasmid-transfected A2780 and normal A2780 cells were used as control (the different four groups were named as WT-PTEN/A2780, C124A-PTEN/A2780, GFP/A2780 and A2780).
RESULTSThe number of penetrating cells was significantly less in WT-PTEN/A2780 cells (24.3 ± 2.5) than those in C124A-PTEN/A2780, GFP/A2780 and A2780 cells (43.7 ± 3.8, 44.7 ± 2.1 and 45.0 ± 3.0) (P < 0.05). The migration distance was markedly shorter in WT-PTEN/A2780 cells (54.1 ± 3.7) than those in C124A-PTEN/A2780, GFP/A2780 and A2780 cells (78.7 ± 3.4, 78.1 ± 3.1 and 76.8 ± 3.5) (P < 0. 05).
CONCLUSIONSTransfection with PTEN may suppress the invasive and migration ability of ovarian cancer cell line A2780 depending on its phosphatase activity, and the suppressive effect may be due to the down-regulation of MMP-9 in the cancer cells.
