Expression of RhoC and ROCK-1 and their effects on MAPK and Akt proteins in prostate carcinoma.
- Author:
Qiang BU
1
;
Hua-ming TANG
;
Jian TAN
;
Xiao HU
;
Dong-wen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Bone Neoplasms; metabolism; secondary; Cell Line, Tumor; Humans; Male; Mitogen-Activated Protein Kinases; metabolism; Neoplasm Grading; Neoplasm Staging; Phosphorylation; Prostatectomy; Prostatic Neoplasms; metabolism; pathology; surgery; Proto-Oncogene Proteins c-akt; metabolism; RNA, Messenger; metabolism; Transfection; Up-Regulation; rho GTP-Binding Proteins; genetics; metabolism; rho-Associated Kinases; genetics; metabolism; rhoC GTP-Binding Protein
- From: Chinese Journal of Oncology 2011;33(3):202-206
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of RhoC and Rho kinase 1 (ROCK-1) in prostate carcinoma, and explore the possible mechanism of RhoC/ROCK-1 in the pathogenesis of prostate carcinoma.
METHODSTissue specimens from 73 patients with prostate carcinoma and corresponding paracancerous tissues were obtained by prostate cancer biopsy or radical prostatectomy. The expression of RhoC/ROCK-1 mRNA was detected by RT-PCR. Western blot and immunohistochemistry were performed to dertect the expression of RhoC/ROCK-1 protein. Eukaryotic expression plasmids of RhoC were constructed and transfected into PC-3M-2B4 cells. p-MAPK and p-Akt were detected by Western bolt.
RESULTSThe expression levels of RhoC and ROCK-1 mRNA in the prostate carcinomas were significantly higher than those in corresponding paracancerous tissues [72.6% (53/73) vs. 34.2% (25/73); 68.5% (50/73) vs. 38.4% (28/73), P < 0.01], respectively. The results indicated that RhoC/ROCK-1 mRNA expression had no significant correlation with Gleason grade. However, the expression of RhoC/ROCK-1 mRNA showed a significant positive correlation with distant metastasis. The RhoC/ROCK-1 protein expression in prostate cancer was also higher than corresponding paracancerous tissues, and showed a significant positive correlation with p-MAPK and p-Akt expression levels. In addition, p-MAPK and p-Akt expression levels were up-regulated in the transcripts.
CONCLUSIONExpression levels of RhoC and ROCK-1 in prostate carcinoma are higher than those in corresponding paracancerous tissues, showing a significant positive correlation with distant metastasis. RhoC/ROCK-1 may be involved in the development, invasion and metastasis of prostate carcinoma.
