Expression of BRAF V600E mutation in different thyroid lesions.
- Author:
Wei WANG
1
;
Xiang-hong LI
;
Yin-hui ZHANG
;
Jing LI
;
Jue CHEN
;
Pan ZHAO
;
Qiao-yun LI
;
Jing-jing XIANG
;
Xiao LI
;
Ru-jun XU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma, Follicular; genetics; metabolism; Adenoma; genetics; metabolism; Adult; Biomarkers, Tumor; genetics; Carcinoma, Papillary; genetics; metabolism; Codon; DNA Mutational Analysis; DNA, Neoplasm; genetics; isolation & purification; Female; Goiter, Nodular; genetics; metabolism; Humans; Male; Middle Aged; Point Mutation; Proto-Oncogene Proteins B-raf; genetics; metabolism; Thyroid Neoplasms; genetics; metabolism
- From: Chinese Journal of Oncology 2011;33(5):354-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the expression of BRAF V600E mutation in 240 Chinese patients with thyroid lesions.
METHODSTwo hundred and forty Chinese patients with thyroid lesions, including 129 papillary thyroid carcinomas (PTC), 12 follicular carcinomas, 4 medullary carcinomas, 30 adenomas, 30 nodular goiters, and 35 papillary hyperplasia. DNA was extracted from thyroid biopsy and paraffin embedded thyroid tissues, and the expression of BRAF V600E mutation was detected by polymerase chain reaction and DNA sequencing assays.
RESULTSThe presence of BRAF V600E mutation was found in 61 of the total group of 240 cases (25.4%). It was only detected in PTC (47.3%), and not detected in other types of malignant and benign thyroid lesions. There was a statistically significant difference between the expression of BRAF V600E mutation in classic type PTC (49.6%) and in follicular type PTC (12.5%,P < 0.05), but statistical data did not show any correlation between BRAF V600E mutation and clinicopathologic parameters in PTC (P > 0.05).
CONCLUSIONSBRAF V600E mutation has a significant correlation with PTC and the detection of BRAF V600E mutation may be used as an important prognostic marker of PTC. Our new method of DNA extraction from paraffin embedded tissues is efficient and inexpensive.
