Effects of JAZF1 overexpression on proinflammatory cytokines in hepatocytes induced by palmitic acid.
- Author:
Rui LIU
1
;
Zhimin LIN
;
Yanjun JIA
;
Gangyi YANG
;
Ling LI
;
Ke LI
;
Lili ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Survival; Chemokine CCL2; metabolism; Cytokines; metabolism; Fatty Liver; Hepatocytes; drug effects; metabolism; Humans; Interleukin-8; metabolism; Neoplasm Proteins; metabolism; Palmitic Acid; pharmacology; RNA, Messenger; metabolism; Tumor Necrosis Factor-alpha; metabolism; Up-Regulation
- From: Chinese Journal of Hepatology 2015;23(12):950-954
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of JAZF1 overexpression on the pro-inflammatory cytokines in hepatic steatosis.
METHODSThe model of hepatic steatosis was established by incubating hepatocytes with palmitic acid (PA) at 0, 0.125, 0.25, 0.5 and 1 mM dose and for 0, 6, 12, 24 and 48 hours, after which recombinant adenovirus expressing JAZF1 (Ad-JAZF1) was introduced to up-regulate expression. Triglyceride level was measured by GOD. Cell viability was detected by CCK-8. The mRNA and protein expression of TNF-alpha, MCP-1, IL-8 and JAZF1 was examined by RT-PCR, ELISA, and western blotting.
RESULTSThe PA-treated hepatocytes showed dose-dependent significant increases in TNF-alpha, MCP-1 and IL-8 mRNA expression for doses up to 0.25 mM; there were no significant increases for the highest doses of 0.5 and 1 mM. The 0.25 mM PA-treated hepatocytes showed time-dependent significant increases in TNF-alpha, MCP-1 and IL-8 mRNA expressions (FTNF-alpha = 26.51, FMCP-1 = 57.20, FIL-8 = 353.85, P less than 0.01), with the maximum level reached at 12 h and followed by a gradual decrease with longer treatment times. JAZF1 mRNA and protein expression was markedly increased in hepatocytes infected with Ad-JAZF1 (P less than 0.01). However, the AP-treated hepatocytes with JAZF1 overexpression showed down-regulation of TNF-alpha, MCP-1 and IL-8 mRNA expression (decreased by 89.69%, 77.68%, and 83.21%, respectively) and secretion (37%, 37% and 41%, respectively, P less than 0.01).
CONCLUSIONStimulation of hepatocytes by the PA fatty acid in vitro promotes mRNA expression of TNF-alpha, MCP-1 and IL-8, but overexpression of JAZF1 inhibits the PA-induced expression and secretion of these factors.