Isolation and identification of a Pp-LIM only A gene expressed during cold acclimation in Physcomitrella patens.
- Author:
Mingming SUN
1
;
Rongcai MA
;
Yikun HE
Author Information
1. College of Elementary Education, Capital Normal University, Beijing 100080, China.
- Publication Type:Journal Article
- MeSH:
Acclimatization;
genetics;
physiology;
Amino Acid Sequence;
Amplified Fragment Length Polymorphism Analysis;
Base Sequence;
Bryopsida;
genetics;
physiology;
Cloning, Molecular;
Cold Temperature;
DNA, Complementary;
genetics;
DNA, Plant;
genetics;
Molecular Sequence Data;
Transcription Factors;
genetics
- From:
Chinese Journal of Biotechnology
2008;24(11):1867-1873
- CountryChina
- Language:Chinese
-
Abstract:
Cold acclimation can improve freezing tolerance. Here cDNA amplified fragment length polymorphism (cDNA-AFLP) was used to isolate differentially expressed cDNAs and a Pp-LIM only A cDNA was isolated and identified in the cold acclimation of Physcomitrella patens. Real-time RT-PCR indicated it is obviously up-regulated at 6 h, 12 h, 24 h, 48 h and 72 h after cold acclimation. After comparing the cDNA with the gene sequence, seven introns and eight exons were identified in the cDNA. The cDNA putatively encodes a protein of 345 amino acid residues and only contains one LIM domain which has highly similarity with the PDZ/LIM domains of the protein family in animals. We proposed that Pp-LIM only A be a new gene coding for the LIM-domain containing protein and enhance stability of cell membrane via their effects on cytoskeleton during cold acclimation in P. patens.
