Rapid Isolation of Adipose Tissue-Derived Stem Cells by the Storage of Lipoaspirates.
10.3349/ymj.2011.52.6.999
- Author:
Young Woo EOM
1
;
Jong Eun LEE
;
Mal Sook YANG
;
In Keun JANG
;
Hyo Eun KIM
;
Doo Hoon LEE
;
Young Jin KIM
;
Won Jin PARK
;
Jee Hyun KONG
;
Kwang Yong SHIM
;
Jong In LEE
;
Hyun Soo KIM
Author Information
1. Cell Therapy and Tissue Engineering Center, Wonju College of Medicine, Yonsei University, Wonju, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Lipoaspirates;
adipose tissue;
mesenchymal stem cell;
proliferation;
differentiation
- MeSH:
5'-Nucleotidase/metabolism;
Adipose Tissue/*cytology;
Adult;
Antigens, CD/metabolism;
Antigens, CD44/metabolism;
Antigens, Thy-1/metabolism;
Cell Differentiation/physiology;
Cells, Cultured;
Female;
Humans;
Immunoblotting;
Immunohistochemistry;
Immunophenotyping;
Mesenchymal Stem Cells/metabolism;
Muscle Development/genetics/physiology;
Osteogenesis/genetics/physiology;
Receptors, Cell Surface/metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Stem Cells/*cytology/metabolism;
Young Adult
- From:Yonsei Medical Journal
2011;52(6):999-1007
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs). MATERIALS AND METHODS: Aliquots (10 g) of the lipoaspirates were stored at 4degrees C without supplying oxygen or nutrients. At the indicated time points, the yield of mononuclear cells was evaluated and the stem cell population was counted by colony forming unit-fibroblast assays. Cell surface markers, stem cell-related transcription factors, and differentiation potentials of ASCs were analyzed. RESULTS: When the lipoaspirates were stored at 4degrees C, the total yield of mononuclear cells decreased, but the stem cell population was enriched. These ASCs expressed CD44, CD73, CD90, CD105, and HLA-ABC but not CD14, CD31, CD34, CD45, CD117, CD133, and HLA-DR. The number of ASCs increased 1x1014 fold for 120 days. ASCs differentiated into osteoblasts, adipocytes, muscle cells, or neuronal cells. CONCLUSION: ASCs isolated from lipoaspirates and stored for 24 hours at 4degrees C have similar properties to ASCs isolated from fresh lipoaspirates. Our results suggest that ASCs can be isolated with high frequency by optimal storage at 4degrees C for 24 hours, and those ASCs are highly proliferative and multipotent, similar to ASCs isolated from fresh lipoaspirates. These ASCs can be useful for clinical application because they are time- and cost-efficient, and these cells maintain their stemness for a long time, like ASCs isolated from fresh lipoaspirates.
