Preparation and identification of recombinant maxadilan.
- Author:
Tianhong YI
1
;
An HONG
;
Shanshan XIE
;
Ling ZHANG
;
Qiuling XIE
;
Yun DAI
;
Rongjie YU
Author Information
1. Bio-engineering Institute of Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Escherichia coli;
genetics;
metabolism;
Insect Proteins;
biosynthesis;
genetics;
Inteins;
genetics;
Isopropyl Thiogalactoside;
pharmacology;
Molecular Sequence Data;
Recombinant Proteins;
analysis;
biosynthesis;
genetics
- From:
Chinese Journal of Biotechnology
2008;24(12):2049-2055
- CountryChina
- Language:Chinese
-
Abstract:
To produce recombinant Maxadilan using gene engineering technology, the gene of recombinant Maxadilan which expressed in protocaryon were designed and synthesized according to the amino acid sequences of Maxadilan. The recombinant plasmid pKYB-MAX was constructed and transformed into host bacteria Escherichia coli strain ER2566. After the MAX-intein-CBD fusion protein was purified by chintin-affinity chromatography, the self-cleavage activity of the intein was induced by beta-mercaptoethanol and the recombinant Maxadilan was released from the chitin-bound intein tag. The molecular weight of peptides was determined by the laser flight mass spectrometry and the results was conformity with the theoretical value. The biological activity analysis showed that recombinant Maxadilan significantly enhanced the concentration of serum glucose.
