Establishment of fibroblast cell line and its biological characteristics in Matou goat.
- Author:
Tianda LI
1
;
Chousheng LIU
;
Zhigang WANG
;
Liping ZHANG
;
Xiuzhu SUN
;
Junjin ZHAO
;
Fei MENG
;
Guihe LUO
;
Jinqing ZHU
Author Information
1. College of Animal Science & Technology, Gansu Agricultural University, Lanzhou 730050, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Cell Movement;
Cell Survival;
China;
Diploidy;
Ear, External;
cytology;
Fibroblasts;
cytology;
Goats;
Karyotyping
- From:
Chinese Journal of Biotechnology
2008;24(12):2056-2060
- CountryChina
- Language:Chinese
-
Abstract:
Taking Matou goat ear margin as the study material, we succeeded in established a fibroblast cell line by the method of explant culture directly. Observations on morphology, dynamic growth, determination of viability, analysis of karyotype, test of microorganism and other characteristics were detected. Results showed: Population Doubling Time (PDT) of cells was approximately 36 h; Cell viability was 96.7% after thawing; The status of cell After passage was constant; Analysis of chromosomal karyotyps indicated that diploid (2n=60) account for 98% in the cell line. Every index in the cell line met all the standard quality controls of ATCC in USA. The established of Matou goat ear fibroblast cell line has not only important genetic resources preserved at the cell level, but also valuable material for genome, postgenome and somatic cell nuclear transfer research.
