Effects of trxS gene on protein degradation in germinating barley seeds.
- Author:
Li WEI
1
;
Weiwei KONG
;
Jun YIN
;
Weijie WANG
Author Information
1. Henan Agricultural University, Zhengzhou 450002, China.
- Publication Type:Journal Article
- MeSH:
Germination;
Hordeum;
genetics;
growth & development;
metabolism;
Peptide Hydrolases;
metabolism;
Plant Proteins;
metabolism;
Plants, Genetically Modified;
genetics;
growth & development;
metabolism;
Seeds;
growth & development;
Thioredoxins;
genetics;
metabolism;
Transformation, Genetic
- From:
Chinese Journal of Biotechnology
2009;25(1):84-88
- CountryChina
- Language:English
-
Abstract:
We assessed the effects of trxS gene on changes of proteinase activity, contents of different protein fractions and SDS-PAGE profiles in germinating seeds of contrasting transgenic and nontransgenic barley variety. Proteinase activity was enhanced by 70.28% in transgenic than nontransgenic barley seeds, whereas contents of albumin, globulin, hordein and glutelin in transgenic seeds were 3.68%, 23.52%, 31.37%, and 21.04%, lower than those in nontransgenic seeds. Degradation rates of hordein and glutelin in transgenic seeds were faster than those in nontransgenic seedlings as indicated by the SDS-PAGE profiles. Our data imply that the transformation of trxS gene could promote the degradation of protein, providing theoretic basis for the use of trxS gene and barley quality breeding.
