Effect of catechin on bone marrow cell cycle and gene expression of hematopoietic growth factors.
- Author:
Ping LIU
1
;
Dong-xiao WANG
;
Ruo-yun CHEN
;
Meng-li CHEN
;
Jian-fen YIN
;
Gui-yun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; metabolism; Catechin; isolation & purification; pharmacology; Cell Cycle; drug effects; Fabaceae; chemistry; Female; Gene Expression Regulation; drug effects; Granulocyte-Macrophage Colony-Stimulating Factor; biosynthesis; genetics; Hematopoietic Stem Cells; cytology; Interleukin-6; biosynthesis; genetics; Male; Mice; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Spleen; cytology; metabolism
- From: Acta Pharmaceutica Sinica 2004;39(6):424-428
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of catechin, the active component of Spatholobus suberectus Dunn, on bone marrow cell cycle and the expression of IL-6 and GM-CSF mRNA in spleen cells of normal and marrow-depressed mice in order to clarify the mechanism of hematopoietic-supportive effect of catechin.
METHODSFlow cytometry was adopted to investigate the influence of catechin on bone marrow cell cycle in mice and the expression of IL-6 and GM-CSF mRNA induced by catechin in spleen cells was detected by RT-PCR technique simultaneously.
RESULTSThe cell proportion of normal and marrow-depressed mice in G0/G1 phase was reduced significantly, while that in S + G2/M phase increased significantly. Being induced by catechin, IL-6 mRNA and GM-CSF mRNA in spleen cells were markedly up-regulated.
CONCLUSIONCatechin (2 g x L(-1), intraperitoneally injected to mice daily immediately after irradiation for 7 consecutive days) was shown to promote the expression of IL-6 mRNA and GM-CSF mRNA in spleen cells of mice, through which it can accelerate bone marrow cells of normal mice into cell cycle and help those of marrow-depressed mice to get out of "G1-phase-block", enter into cell cycle and radically accelerate the proliferation and differentiation of hematopoietic stem cell/hematopoietic progenitor cell (HSC/HPC).
