Biotransformation of dehydroepiandrosterone by hairy root cultures of Anisodus tanguticus.
- Author:
Ying LIU
1
;
Ke-di CHENG
;
Ping ZHU
;
Wen-hua FENG
;
Chao MENG
;
Hui-xin ZHU
;
Hui-xia HE
;
Xiao-jun MA
Author Information
- Publication Type:Journal Article
- MeSH: Androstenedione; chemistry; isolation & purification; Androstenes; chemistry; isolation & purification; Biotransformation; Culture Media; Dehydroepiandrosterone; chemistry; metabolism; Molecular Structure; Plant Roots; metabolism; Plants, Medicinal; metabolism; Solanaceae; metabolism; Tissue Culture Techniques
- From: Acta Pharmaceutica Sinica 2004;39(6):445-448
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo modify the structure of dehydroepiandrosterone (DHEA).
METHODSUsing hairy root cultures of Anisodus tanguticus to perform biotransformation of DHEA, using chromatographic and spectral techniques to isolate and identify the products.
RESULTS(1) The MS medium without plant hormone was suitable for the growth of the hairy root. (2) DHEA was converted into five products: androst-4-ene-3, 17-dione (I); 6alpha-hydroxyandrost-4-ene-3, 17-dione (II); 6alpha, 17beta-dihydroxyandrost-4-ene-3-one (III); androst-4-ene-3, 6, 17-trione (IV) and 17beta-hydroxyandrost-4-ene-3-one (V).
CONCLUSIONIt is the first time to use hairy root cultures of Anisodus tanguticus for the biotransformation of DHEA and five DHEA-related compounds were obtained.
