Effects of puerarin on renal function, expressions of MMP-2 and TIMP-2 in diabetic rats.
- Author:
Hui-Jun DUAN
1
;
Shu-Xia LIU
;
Yu-Jun ZHANG
;
Qing-Juan LIU
;
Ning HE
;
Ying-Min LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Collagen Type IV; metabolism; Diabetic Nephropathies; chemically induced; metabolism; physiopathology; Isoflavones; pharmacology; Kidney; enzymology; metabolism; pathology; Laminin; metabolism; Male; Matrix Metalloproteinase 2; biosynthesis; genetics; Peptide Fragments; metabolism; Protective Agents; pharmacology; RNA, Messenger; biosynthesis; Rats; Rats, Wistar; Streptozocin; Tissue Inhibitor of Metalloproteinase-2; biosynthesis; genetics; Transforming Growth Factor beta; metabolism; Transforming Growth Factor beta1
- From: Acta Pharmaceutica Sinica 2004;39(7):481-485
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of puerarin on expressions of MMP-2 and TIMP-2 in the kidney of diabetic rats.
METHODSUninephrectomized male Wistar rats were used to induce diabetes by intraperitoneal injection of streptozocin (65 mg x kg(-1)). Puerarin was given daily by intraperitoneal injection from the third day of induction of diabetes for 16 weeks. Using in situ hybridization and immunohistochemistry to detect MMP-2, TIMP-2 mRNA expressions and MMP-2, TIMP-2, collagen IV and Laminin expressions in diabetic kidneys with image analysis system, Flow cytometry was used to detect the expressions of TGFbeta1, MMP-2 and TIMP-2.
RESULTSCompared with those in kidneys of control group, expressions of MMP-2 mRNA and proteins were lower, while the expressions of both TGFbeta1 and TIMP-2 were higher in the diabetic kidney (P < 0.05). The level of MMP-2 expression was advanced, while expression of TIMP-2 was reduced by puerarin treatment (P < 0.05).
CONCLUSIONPuerarin showed some renal protective effect on diabetic nephropathy, partly through inhibition of excessive deposition of glomeruli extracellular matrix by up-regulating MMP-2 and down-regulating TIMP-2 expressions besides reducing the blood glucose.
