Mechanisms of sodium ferulate inhibition of collagen synthesis in hepatic stellate cells.
- Author:
Jin HUANG
1
;
Jin-hong HU
;
Lei QIU
;
Zhen CAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Collagen; biosynthesis; Coumaric Acids; pharmacology; Epithelial Cells; cytology; Hepatocytes; cytology; metabolism; Lung; cytology; Mink; Oxidative Stress; Rats; Transforming Growth Factor beta; metabolism; Transforming Growth Factor beta1
- From: Acta Pharmaceutica Sinica 2004;39(8):577-580
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the mechanisms of sodium ferulate (SF) on inhibition of collagen synthesis in hepatic stellate cells.
METHODSCollagen synthesis was analyzed by measuring 3H-proline incorporation. ELISA method was used to study the effect of SF on transforming growth factor beta1 (TGFbeta1) level in cultured HSC-T6 cell. The effect of SFon the TGFbeta1 activity in the supernatant of culture was analyzed by mink lung epithelial cell (Mv1Lu) proliferation inhibition by MTT assay.
RESULTSSF inhibited collagen synthesis in hepatic stellate cells stimulated with TGFbeta1. SF was shown to decrease TGFbeta1 level in the supernatant of HSC-T6 increased by oxidative stress. TGFbeta1 activity was intervened by SF.
CONCLUSIONSF could decrease collagen synthesis, with mechanism may be associated with that SF intervened TGFbeta1 activity, and reduced the level of TGFbeta1 increased by oxidative stress.
