Determination of progesterone and its main metabolite in rat plasma and uterus using HPLC.
- Author:
Li-wen ZHANG
1
;
Ruo-bing CHAO
Author Information
- Publication Type:Journal Article
- MeSH: 20-alpha-Dihydroprogesterone; blood; pharmacokinetics; Animals; Area Under Curve; Chromatography, High Pressure Liquid; methods; Female; Injections, Intramuscular; Progesterone; blood; metabolism; pharmacokinetics; Rats; Rats, Wistar; Uterus; metabolism
- From: Acta Pharmaceutica Sinica 2004;39(8):613-617
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo quantify progesterone (P) and one of its metabolites 20alpha-hydroxy-4-pregnen-3-one (20alpha-OHP) in rat plasma and uterus after im administration of progesterone.
METHODSPlasma and uterus samples were prepared by liquid-liquid extraction and separated through Shimadzu VP-ODS column (150 mm x 4.6 mm ID, 5 microm). The mobile phase consisted of acetonitrile and water (60: 40, adjusted to pH 4.0 with phosphoric acid). The detector was set at 240 nm. Norgestrel was used as the internal standard.
RESULTSCmax of P in plasma was (508 +/- 62) microg x L(-1), Tmax was (3.2 +/- 0.4) h, T1/2 (ke) was (10 +/- 4) h and mean AUC0-48h was (5886 +/- 1573) microg x L(-1) x h. The maximum concentration of P in uterus was (1.7 +/- 1.1) microg x g(-1) and the peak time was (5.2 +/- 1.11) h. 20alpha-OHP showed a similar Tmax with P.
CONCLUSIONThe method is accurate and convenient. It can be used to determine P and its main metabolite 20alpha-OHP simultaneously for studying their preclinical pharmacokinetics.
