Application of PSD-MALDI-TOF-MS to protein identification.
- Author:
Jie WANG
1
;
Song-cheng YANG
;
Sheng-ming WU
;
Hong-xia WANG
;
Kai-hua WEI
;
Xue-min ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adrenocorticotropic Hormone; chemistry; Amino Acid Sequence; Databases, Protein; Electrophoresis, Gel, Two-Dimensional; Molecular Sequence Data; Peptide Fragments; chemistry; Peptide Mapping; Ribosomal Proteins; analysis; chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tissue Plasminogen Activator; chemistry
- From: Acta Pharmaceutica Sinica 2004;39(8):627-630
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search.
METHODSThe PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA.
RESULTSTwo unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method.
CONCLUSIONPSD technique has greater application prospects in peoteomics.
