Effect of ginsenoside Rg1 on expression of p21, cyclin E and CDK2 in the process of cell senescence.
- Author:
Chao-hui ZHAO
1
;
Xiao-chun CHEN
;
Jian-sheng JIN
;
Yuan-gui ZHU
;
Guang-bin SHI
;
Yu-qi ZENG
;
Yong-kun LI
;
Xu PENG
Author Information
- Publication Type:Journal Article
- MeSH: CDC2-CDC28 Kinases; metabolism; Cell Line; Cellular Senescence; drug effects; Cyclin E; metabolism; Cyclin-Dependent Kinase 2; Fibroblasts; cytology; metabolism; Ginsenosides; isolation & purification; pharmacology; Humans; Panax; chemistry; Plants, Medicinal; chemistry; Proto-Oncogene Proteins p21(ras); metabolism; tert-Butylhydroperoxide; antagonists & inhibitors
- From: Acta Pharmaceutica Sinica 2004;39(9):673-676
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore the possible role of p21, cyclin E and cyclin-dependent kinase 2 (CDK2) in the protection of ginsenoside Rg1 against tert-butylhydroperoxide (t-BHP)-induced senescence in WI-38 cells.
METHODSThe cellular ultrastructure, cytometric assay and beta-galactosidase (beta-gal) cytochemistry staining were used to evaluate cell senescence. The levels of of p21, cyclin E and CDK2 protein were detected by Western blot.
RESULTSPretreatment with Rg1 significantly attenuated t-BHP-induced senescence in WI-38 cells. Simultaneously, compared with cells treated with t-BHP alone, Rg1 pretreatment markedly decreased the level of p21 protein and increased the levels of CDK2 and cyclin E.
CONCLUSIONp21, cyclin E and CDK2 may be involved in the process of ginsenoside Rg1 protection against t-BHP-induced senescence in WI-38 cells.
