Effect of 17beta-estradiol on Kv2.1 current and delayed rectifier potassium current in cultured rat hippocampal neurons.
- Author:
Wei ZHANG
1
;
Hong-wei JIN
;
Hai-xia ZHANG
;
Shao-feng XU
;
Hai-bo YU
;
Xiao-liang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Line; Cells, Cultured; Delayed Rectifier Potassium Channels; Dose-Response Relationship, Drug; Embryo, Mammalian; Estradiol; pharmacology; Female; Hippocampus; cytology; physiology; Humans; Kidney; cytology; Male; Neurons; cytology; physiology; Patch-Clamp Techniques; Potassium Channels, Voltage-Gated; drug effects; Rats; Rats, Wistar; Shab Potassium Channels
- From: Acta Pharmaceutica Sinica 2004;39(9):686-690
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of 17beta-estradiol on Kv2.1 potassium channel current and delayed rectifier potassium current (IK) in cultured rat hippocampal neurons.
METHODSThe effects of 17beta-estradiol on Kv2.1 channel current and IK in cultured rat hippocampal neurons were observed using the whole cell patch clamp techniques.
RESULTS17beta-Estradiol was shown to reduce the amplitude of Kv2.1 current and IK in concentration-dependent manners. The IC50s of 17beta-estradiol blocking Kv2.1 and IK were 2.4 and 4.0 micromol x L(-1), respectively. 17beta-Estradiol (3 micromol x l(-1)) significantly shifted the steady-state activation and inactivation curves of Kv2.1 current to negative potentials. However, it only produced the shift of the steady-state activation curve of IK to the negative potential without effect on the steady-state inactivation of IK.
CONCLUSION17beta-Estradiol inhibits Kv2.1 and IK of hippocampus at similar level. The inhibition of 17beta-estradiol on IK current may be partially via blocking Kv2.1 current.
