Genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.

Yun Wang; Zhangjian Chen; Te BA; Ji PU; Yongen GU; Jian Guo; Guang Jia

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Genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.

Author: Yun WANG ¹ ; Zhangjian CHEN ¹ ; Te BA ¹ ; Ji PU ¹ ; Yongen GU ¹ ; Jian GUO ¹ ; Guang JIA ²
Author Information

1. Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University Health Science Center, Beijing 100191, China.
2. Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University Health Science Center, Beijing 100191, China. Email:jiaguangjia@bjmu.edu.cn.
Publication Type:Journal Article
MeSH: Animals; Bone Marrow Cells; DNA Damage; Histones; Male; Micronuclei, Chromosome-Defective; Nanoparticles; Rats; Titanium
From: Chinese Journal of Preventive Medicine 2014;48(9):815-818
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.
METHODSTwenty-eight SD male young rats (4 weeks old) were randomly divided into 4 groups, which were exposed to TiO2 nanoparticles ((75 ± 15) nm, anatase) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight (bw) every day for 30 days. The bone marrow cells were collected for micronuclei and γ-H2AX immunofluorescence analysis.
RESULTSThe percentage of γ-H2AX foci-positive cells (37.4 ± 10.0)% in the 50 mg/kg bw dose group were significantly higher than that in the control group (19.8 ± 3.1)% (t value was -17.59, P < 0.01). No significantly difference was found in polychromatic erythrocyte/normochromatic erythrocyte (PCE/NCE) ratio and PCE micronucleus rate between three experimental groups and control group.
CONCLUSIONTiO2 nanoparticles can increase the frequency of DNA double-strand breaks in bone marrow cells, but has no effect on micronucleus of bone marrow cells in young rats.