Slow rise of intracellular Ca(2+) concentration in rat primary sensory neurons triggered by loureirin B.
- Author:
Yi-Ning YANG
1
;
Jue-Xu CHEN
;
Xue-Yan PANG
;
Susumu TERAKAWA
;
Xu CHEN
;
Yong-Hua JI
;
Ke-Lan YONG
Author Information
1. Shanghai University, Shanghai, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Caffeine;
pharmacology;
Calcium;
metabolism;
Ganglia, Spinal;
drug effects;
metabolism;
Neurons, Afferent;
drug effects;
metabolism;
Rats;
Resins, Plant;
pharmacology
- From:
Acta Physiologica Sinica
2009;61(2):115-120
- CountryChina
- Language:English
-
Abstract:
In the present study, the intracellular free calcium concentration ([Ca(2+)](i)) in acutely isolated rat dorsal root ganglia (DRG) neurons modulated by loureirin B, an active component of "dragon's blood" which is a kind of Chinese herbal medicine, was determined by the means of Fura-2 based microfluorimetry. It was found that loureirin B could evoke the elevation of [Ca(2+)](i) in a dose-dependent manner. However, the elevation of [Ca(2+)](i) evoked in the calcium free solution was much smaller than that in the standard external cell solution, suggesting that most change of [Ca(2+)](i) was generated by the influx of extracellular Ca(2+), not by the activities of intracellular organelles like Ca(2+) stores and mitochondria. In addition, the mixture of loureirin B and caffeine also induced [Ca(2+)](i) rise, but the peak of [Ca(2+)](i) rise induced by the mixture was significantly lower than that by caffeine alone, which means the triggering pathway and the targets of caffeine are probably involved in loureirin B-induced [Ca(2+)](i) rise. Moreover, compared to the transients induced by caffeine, KCl and capsaicin, the loureirin B-induced [Ca(2+)](i) rise is much slower and more stable. These results indicate that the capability of loureirin B of inducing the [Ca(2+)](i) rise is solid and unique.
