Suppression of gamma-aminobutyric acid transporter current by activation of ionotropic glutamate receptors on retinal horizontal cells.
- Author:
Xiao-Dong JIANG
1
;
Yan SUN
;
Xu-Long WANG
;
Hai-Qing GONG
;
Pei-Ji LIANG
Author Information
1. School of Life Sciences and Biotechnology, Shanghai Jiaotong University, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Carps;
Egtazic Acid;
analogs & derivatives;
pharmacology;
GABA Plasma Membrane Transport Proteins;
metabolism;
Receptors, Ionotropic Glutamate;
metabolism;
Retinal Horizontal Cells;
metabolism;
gamma-Aminobutyric Acid;
pharmacology
- From:
Acta Physiologica Sinica
2009;61(4):299-304
- CountryChina
- Language:English
-
Abstract:
In the present study, the modulatory effect of AMPA receptors on gamma-aminobutyric acid (GABA) transporter current was investigated on enzymatically isolated horizontal cells of carp retina. The GABA transporter current elicited by 1 mmol/L GABA was decreased immediately after pre-application of AMPA (30 mumol/L or 3 mmol/L) for 50 s. Application of 10 mmol/L BAPTA in intracellular solution inhibited the suppression effect of AMPA on GABA transporter current. The suppression effect induced by co-application of 3 mmol/L AMPA and 3 mmol/L NMDA was similar to that of 3 mmol/L AMPA or 3 mmol/L NMDA alone. These results suggest that the activation of AMPA receptors inhibits GABA transporter-mediated current by affecting intracellular Ca(2+) processes in the retinal horizontal cells, which is identical with the modulatory effect of NMDA receptors on GABA transporters.
