Effect of 5-aza-2'-deoxycytidine on DAPK gene expression in human HL-60 cells.
10.7534/j.issn.1009-2137.2014.03.027
- Author:
Chun-Yan WANG
1
;
Wen-Jun LIU
2
Author Information
1. Department of Pediatrics, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
2. Department of Pediatrics, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China. E-mail: lwjlyfy@sina.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Death-Associated Protein Kinases;
genetics;
Deoxycytidine;
pharmacology;
Gene Expression;
drug effects;
HL-60 Cells;
Humans
- From:
Journal of Experimental Hematology
2014;22(3):717-722
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of methylation transferase inhibitor 5-aza-2'-deoxycytidine (5-aza-2dC) of different concentrations on the apoptosis of human acute myeloid leukemia (AML) cell line HL-60 and the expression of DAPK gene in HL-60 cells, as well as to explore the possible anti-AML mechanism of 5-aza-2dC. HL-60 cells were treated by 5-aza-2dC of different concentrations. The effect of 5-aza-2dC on the HL-60 cell morphology was observed by Wright's staining. The effect of 5-aza-2dC on HL-60 cell apoptosis and DAPK mRNA expression was detected by flow cytometry and reverse transcription-polymerize chain reaction (RT-PCR) respectively. The results showed that the 5-aza-2dC induced the apoptosis of HL-60 cells in a concentration-dependent manner; the 5-aza-2dC increased the expression levels of DAPK mRNA in HL-60 cells in a concentration-dependent manner. It is concluded that the apoptosis rate of HL-60 cells and DAPK mRNA expression level displayed a rising trend with 5-aza-2dC concentration increasing. Therefore, DAPK gene may participate in HL-60 cell apoptosis induced by 5-aza-2dC.
