Effects of rapamycin on biological characteristics of bone marrow mesenchymal stem cells from patients with aplastic anemia.
10.7534/j.issn.1009-2137.2014.03.035
- Author:
Xin WANG
1
;
Feng-Xia MA
1
;
Shi-Hong LU
1
;
Ying CHI
1
;
Fang CHEN
1
;
Xue LI
1
;
Juan-Juan LI
1
;
Wen-Jing DU
1
;
Ying FENG
1
;
Jun-Jie CUI
1
;
Bao-Quan SONG
1
;
Zhong-Chao HAN
2
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. E-mail: hanzhongchao@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Anemia, Aplastic;
metabolism;
Apoptosis;
drug effects;
Autophagy;
Bone Marrow Cells;
cytology;
drug effects;
Cell Cycle;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Humans;
Mesenchymal Stromal Cells;
cytology;
drug effects;
Signal Transduction;
Sirolimus;
pharmacology
- From:
Journal of Experimental Hematology
2014;22(3):762-766
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effects of rapamycin on biological function and autophagy of bone marrow mesenchymal stem cells (BM-MSC) from patients with aplastic anemia so as to provide experimental basis for the clinical treatment of aplastic anemia (AA) with rapamycin. BM-MSC were treated with different concentrations of rapamycin (0, 10, 50, 100 nmol/L) for 48 h, the expression of LC3B protein was detected by Western blot to observe the effect of rapamycin on cell autophagy; cell apoptosis and cell cycles were detected by flow cytometry; the proliferation of BM-MSC of AA patients was measured by cell counting kit-8; the adipogenic differentiation of BM-MSC were tested by oil red O staining after adipogenic induction for 2 weeks; the adipogenic related genes (LPL, CFD, PPARγ) were detected by real-time PCR. The results showed that the proliferation and adipogenesis of BM-MSC of AA patients were inhibited by rapamycin. Moreover, the autophagy and apoptosis of BM-MSC were increased by rapamycin in a dose-dependent way.Rapamycin arrested the BM-MSC in G0/G1 phase and prevented them into S phase (P < 0.05). It is concluded that rapamycin plays an critical role in inhibiting cell proliferation, cell cycles, and adipogenesis, these effects may be related with the autophagy activation and mTOR inhibition resulting from rapamycin.
