Anti-leukemia mechanism of miR-17 and miR-20a silencing mediated by miRNA sponge.
10.7534/j.issn.1009-2137.2014.04.010
- Author:
Wen-Yan NIU
1
,
2
;
Shun-Quan WU
1
;
Zhen-Zhen XU
1
;
Jun LIN
1
;
Rong ZHAN
1
;
Author Information
1. Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital
2. Fuzhou 350001, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Gene Silencing;
Genetic Vectors;
Humans;
Leukemia;
genetics;
pathology;
Leukemia, Myeloid, Acute;
genetics;
MicroRNAs;
genetics
- From:
Journal of Experimental Hematology
2014;22(4):932-937
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to quantitatively detect the expression levels of pre-miR-17 and pre-miR-20a in acute leukemia patients and eight kinds of leukemia cell lines, and to investigate the anti-leukemia mechanism of miR-17 and miR-20a silence mediated by miRNA Sponge. Quantitative real-time PCR was used to detect the mRNA expression levels of pre-miR-17 and pre-miR-20a in patients with various types of leukemia and leukemia cell lines. The Jurkat cells over-expressing miR-17 and miR-20a were transfected with recombinant lentivirus-transfecting units targeted at miR-17 and miR-20a plus 6 µg/ml of polybrene. Then the proliferation ability and cell cycle of Jurkat cells was evaluated by CCK-8 and flow cytometry respectively. The results showed that the expression level of pre-miR-17 and pre-miR-20a in all leukemia patients was significantly higher than that in normal group(P < 0.05), the expression of pre-miR-17 and pre-miR-20a in acute lymphoid leukemia was significantly higher than that in acute myeloid leukemia(P < 0.05), and the pre-miR-17 and pre-miR-20a expression level did not correlate significantly with high white blood cell count>20.0×10(9)/L(P > 0.05). The miR-17 and miR-20a silencing mediated by miRNA Sponge led to a significant decrease of cell growth, restored G1 accumulation and increase of cell apoptosis. It is concluded that the expression of miR-17 and miR-20a is upregulated in leukemia patients, which may contribute to leukemogenesis. Over-expressed miR-17 and miR-20a promote cell growth and cell cycle progression, and inhibit apoptosis through negatively-regulating P21 and E2F1 after-transcriptionally.
