Inhibitory effect of genistein on the proliferation of Raji cells and its related mechanism.
10.7534/j.issn.1009-2137.2014.04.016
- Author:
Jing SHEN
1
;
Jing-Shi WANG
1
;
Li FU
1
;
Da-Yong HUANG
1
;
Zhao WANG
2
Author Information
1. Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China.
2. Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China. E-mail: zhaowww263@yahoo.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Dose-Response Relationship, Drug;
Genistein;
pharmacology;
Humans;
Lymphocytes;
metabolism;
Nuclear Receptor Co-Repressor 1;
metabolism;
Proto-Oncogene Proteins c-bcl-6;
metabolism
- From:
Journal of Experimental Hematology
2014;22(4):971-975
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the anti-proliferative effect of genistein (Gen) on BCL-6 positive Raji cells and its related mechanism. Trypan blue staining and MTT method were used to analyze the anti-proliferative effect of Gen on Raji cells. Cell apoptosis, protein expression and the interaction of BCL-6 and NCoR were detected by PI/AV dual staining, Western blot and Co-IP method, respectively. The results showed that Gen had time- and dose-dependent inhibitory effect on Raji cell proliferation and induced apoptosis. Different dose of Gen had no significant effect on the expression of BCL-6 and NCoR, but could inhibit the binding of BCL-6 and NCoR. It is concluded that Gen shows inhibitory effect on BCL-6 positive lymphoma cells, which can be as a adjuvant therapy for combined rituximab with chemotherapy.
