Effects of salidroside on proliferation of bone marrow mesenchymal stem cells.
10.7534/j.issn.1009-2137.2014.04.035
- Author:
Hai BAI
1
;
Cun-Bang WANG
2
;
Xiao-Hui MA
2
;
Yu-Ping WEI
2
;
Rui XI
2
;
Qiang ZHAO
2
;
Qian ZHANG
2
Author Information
1. Department of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China. E-mail: baihai98@hotmail.com.
2. Department of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
cytology;
drug effects;
Cell Cycle;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Glucosides;
pharmacology;
Humans;
Mesenchymal Stromal Cells;
cytology;
drug effects;
Phenols;
pharmacology;
Stem Cell Factor;
secretion
- From:
Journal of Experimental Hematology
2014;22(4):1072-1077
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of salidroside on proliferation of bone marrow mesenchymal stem cells (MSC) and their secretion of stem cell factor (SCF). MSC were isolated and amplified in vitro via density gradient centrifugation and adherence screening method. MCS were identified by flow cytometry and osteogenic/adipogenic induction. The effects of salidroside on cell proliferation, cell cycle and the SCF secretion of MSC were detected by flow cytometry. The results showed that the salidroside could induce the proliferation of MSC, peaked at the concentration of 1.5 mg/ml and in a time-dependent manner (in 24 h, 48 h and 72 h). Salidroside at 1.5 mg/ml could more effectively increase the percentage of cells in S and G1/M phase. Co-cultured with salidroside at the concentration of 1.5 mg/ml for 48 h, the SCF and the expression levels of SCF mRNA in co-culture supernatant were both significantly increased (P < 0.01). It is concluded that salidroside in a range of certain concentration can obviously promote the proliferation of MSC and increase the expression and secretion of SCF.
