Protective effect of bone marrow mesenchymal stem cell-derived microvesicles on glutamate injured PC12 cells.
10.7534/j.issn.1009-2137.2014.04.036
- Author:
Shan-Shan LIN
1
;
Bo ZHU
2
;
Zi-Kuan GUO
3
;
Guo-Zhi HUANG
4
Author Information
1. Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
2. Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
3. Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, China.
4. Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China. E-mail: drhuang66@gmail.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Bone Marrow Cells;
cytology;
Cell Survival;
Coculture Techniques;
Cytoplasmic Vesicles;
Flow Cytometry;
Glutamic Acid;
adverse effects;
Mesenchymal Stromal Cells;
cytology;
PC12 Cells;
Rats;
Receptors, Glutamate;
metabolism
- From:
Journal of Experimental Hematology
2014;22(4):1078-1083
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the protective effect of bone mesenchymal stem cell-derived microvesicles (BMMSC-MV) on glutamate injured PC12 cells so as to elucidate the mechanism of the neural damage repair. BMMSC were isolated and purified with density-gradient centrifugation method, BMMSC-MV were harvested from the supernatants of BMMSC by hypothermal ultracentrifugation method. The surface markers of BMMSC reacted against different antibodies were detected by flow cytometry. The morphology features of MV were observed under an electron microscope. Experiment was divided into three groups, one was a control group, and the other two were glutamate-injured group and co-culture group of BMMSC-MV and glutamate-damaged cells respectively. MTT test was used to evaluate the proliferative status of PC12 cells and the AnnexinV-FITC detecting kit and Hoechst33342 were used to detect the apoptosis of PC12 cells in different groups. The results showed that BMMSC isolated from rat bone marrow were highly positive for CD29, CD44 and negative for CD31, CD34 and CD45. The morphology of MV was round and the vesicles were homogenous in size. BMMSC-MV exhibited a protective effect on the excitotoxicity-injured PC12 cells, displaying increase of cell viability, decrease of Annexin-V/PI staining positive and nuclear condensed cells. It is concluded that BMMSC-MV can protect PC12 cells from glutamate-induced apoptosis, suggesting that BMMSC-MV may be a potential candidate for treatment of neurological diseases.This study provides the preliminary experimental and theoretical evidence for use of BMMSC-MV in treatment of neural excited damage.
