Comparative analysis of collecting mononuclear cells from peripheral blood by using Fenwal CS-3000 plus, haemonetics MCS plus and COBE spectra separators.
10.7534/j.issn.1009-2137.2014.04.041
- Author:
Wen-Ling ZHOU
1
;
Yi-Wen HAO
2
Author Information
1. Department of Blood Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. E-mail: zhouweiling0214@126.com.
2. Department of Blood Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
- Publication Type:Journal Article
- MeSH:
Aged;
Cell Separation;
methods;
Cytokine-Induced Killer Cells;
cytology;
Dendritic Cells;
cytology;
Female;
Humans;
Leukapheresis;
instrumentation;
Male;
Middle Aged
- From:
Journal of Experimental Hematology
2014;22(4):1103-1108
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to compare the collection efficiency of mononuclear cells (MNC) from peripheral blood as well as the changes of blood-related indices in patient by using 3 cell separators. MNC were collected from 94 tumor patients by using Fenwal CS-3000plus, Haemonetics MCSplus and COBE spectra separators. Routine blood test was performed before and after MNC collection to detect the potential effects of cell separators on blood-related indices in the patients. MNC count was performed. The percentages of CD3(+), CD4(+) and CD8(+) in peripheral blood cells were determined. The results showed that the MNC counts were (3.08 ± 0.79)×10(9), (3.21 ± 1.12)×10(9), and (3.22 ± 1.84)×10(9) per bag by CS-3000plus, MCSplus and COBE spectra, respectively. And the corresponding decrease of platelet percentage was (6.86 ± 5.70)%, (8.05 ± 5.14)% and (5.89 ± 4.48)%, respectively. The CD3, CD4 and CD8 ratios in peripheral blood of patients before and after treatment were significantly statistical different (P < 0.001). It is concluded that the MNC collection can be performed successfully with CS-3000plus, MCSplus and COBE spectra, and their collections can meet the needs in clinic.
