Effect of different irradiation doses on the establishment of murine cGVHD model after MHC matched spleen stem cell transplantation.
10.7534/j.issn.1009-2137.2014.04.043
- Author:
Xi-Mei LI
1
,
2
;
Heng ZHU
3
;
Bo TANG
1
;
Ya-Nan CHU
4
;
Yuan-Lin LIU
3
;
Fan ZHOU
5
;
Yi ZHANG
6
Author Information
1. Training Base for Postgraduater, Shengyang Military Command General Hospital, Liaoning Medical College, Shenyang 110016, Liaoning Province, China
2. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
3. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
4. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China
5. Training Base for Postgraduater, Shengyang Military Command General Hospital, Liaoning Medical College, Shenyang 110016, Liaoning Province, China. E-mail: zhoufan611@sina.com.cn.
6. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: zhangyi612@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Disease Models, Animal;
Graft Survival;
radiation effects;
Graft vs Host Disease;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred DBA;
Radiation Dosage;
Spleen;
cytology;
Stem Cell Transplantation;
Transplantation Conditioning;
Transplantation, Homologous;
Whole-Body Irradiation
- From:
Journal of Experimental Hematology
2014;22(4):1115-1119
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of different irradiation doses on the establishment of murine cGVHD model after MHC matched spleen stem cell transplantation. The male mouse BALB/c(H)-2d was totally irradiated with different radiation dose of (60)Co (TBI), then was infused with the same number of splenocytes from MHC matched DBA/2 male mice. After transplantation, the bodyweight, general appearance, hair changes, survival time and pathological damage were observed. The results indicated that compared to the control group (0 Gy) and the 7.0 Gy group, the mice irradiated with 7.5 Gy and 8.0 Gy showed cGVHD symptoms and obvious pathological damage. At the end of experiments (60 d after transplantation), all mice irradiated by 7.5 Gy survived while only 60% animals survived in the 8.0 Gy group. It is concluded that under infusion of 10(8) MHC matched splenocytes per mouse, 7.5 Gy irradiation is appropriate to efficiently establish cGVHD model. This study laid an important foundation for further studying the pathogenesis, biological characteristics, and intervention factors of cGVHD.
