Adenovirus-mediated TGF-beta(1) gene transfer to human degenerative lumbar intervertebral disc cells.
- Author:
Jian ZHAO
1
;
Lianshun JIA
;
Deyu CHEN
;
Jian XIAO
;
Xin PAN
;
Xin JING
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Adult; Female; Gene Transfer Techniques; Humans; Intervertebral Disc; cytology; Lumbar Vertebrae; Male; Middle Aged; Transforming Growth Factor beta; genetics; Transforming Growth Factor beta1
- From: Chinese Medical Journal 2002;115(3):409-412
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo test the possibility of modification of human degenerative lumbar disc cells by the exogenous growth factor gene, transforming growth factor beta(1) (TGF-beta(1)) cDNA, and the expression of the encoded protein.
METHODSNucleus pulposus samples were surgically obtained from 8 patients with degenerative lumbar disc disease. The cells were cultured and directly infected by two adenoviral constructs, Ad/CMV-EGFP containing the enhanced green fluorecence protein (EGFP) gene (marker gene) and Ad/CMV-TGF-beta(1) containing the potentially therapeutic TGF-beta(1) gene. Transgene expression was analyzed by fluorescence production and immunohistochemical staining (Ad/CMV-TGF-beta(1)).
RESULTSCulture cells transduced by Ad/CMV-EGFP showed specific green fluorescence under the fluoroscope and expression sustained for at least 4 weeks. When infected by Ad/CMV-TGF-beta(1), approximately 30% of cultured cells were stained brown (+) with TGF-beta(1) staining.
CONCLUSIONThis study established the strategy of delivering a potentially therapeutic gene, TGF-beta(1), by using an adenoviral vector to human degenerative lumbar intervertebral disc cells.
