Development of a rat C6 brain tumor model.
- Author:
Xiang ZHANG
1
;
Jingwen WU
;
Dakuan GAO
;
Zhou FEI
;
Yan QU
;
Junjie JING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Neoplasms; pathology; Disease Models, Animal; Glioma; pathology; Magnetic Resonance Imaging; Rats
- From: Chinese Medical Journal 2002;115(3):455-457
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo establish rat C6 brain-tumor models and find a simple reliable index to judge tumor volume.
METHODSC6 cell suspension (10 microl) containing 10 g/L agarose and 1 x 10(6) cells was injected into the right caudate nucleus of the rat brain by a stereotaxic method. After implantation, rats were observed and given MRI scans. Rats were perfused with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and before natural death. All brains, lungs, spinal cords and tumors were sectioned and inspected. Tumor-containing samples were prepared histologically by hematoxylin and eosin (HE) stains.
RESULTSFifty implanted rats had 100% yield of intracerebral growth, with distant metastasis of 0% to 4%.
CONCLUSIONA rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size.
