Identification of a gene associated with astragalus and angelica's renal protective effects by silver staining mRNA differential display.
- Author:
Ling YU
1
;
Youyong LU
;
Jingzi LI
;
Haiyan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Angelica sinensis; Animals; Astragalus Plant; Drugs, Chinese Herbal; pharmacology; Kidney; drug effects; metabolism; Kidney Diseases; drug therapy; metabolism; Male; Phytotherapy; Polymerase Chain Reaction; Protective Agents; pharmacology; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Staining and Labeling
- From: Chinese Medical Journal 2002;115(6):923-927
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo identify genes associated with the chronic progression of renal disease and a stragalus and angelica (A&A)'s renal protective effects.
METHODSThe technique of silver staining mRNA differential display (DD) was used to investigate changes of gene expression in normal, sclerotic and A&A treated sclerotic kidneys. We isolated genes differentially expressed during the progression of renal disease which could be normalized by A&A.
RESULTSSeveral genes related to A&A's protective effects were isolated and one of them was confirmed by Northern blot.
CONCLUSIONSilver staining mRNA differential display is a simple and effective technique for isolating differentially expressed genes. The isolated new gene may be related to the progression of chronic renal disease and contribute to A&A's protective effects.
