Plating densities, alpha-difluoromethylornithine effects and time dependence on the proliferation of IEC-6 cells.
- Author:
Zili ZHANG
1
;
Weiwen CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Cell Count; Cell Division; drug effects; Cell Line; Eflornithine; pharmacology; Time Factors
- From: Chinese Medical Journal 2002;115(4):518-520
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo characterize the role of plating densities and alpha-difluoromethylornithine (DFMO) on the proliferation of IEC-6 cells in vitro.
METHODSIEC-6 cells were seeded in 96-well microplates at various densities in the presence or absence of DFMO. Cells were counted and their proliferative capability was monitored Days 1 to 7 with MTT assay at an optical density of 570 nm.
RESULTSThere was a positive relationship between cell number and OD value (r = 0.954, P < 0.01). Higher plating densities (> 0.5 x 10(4) cells/well) inhibited the growth of cells on Day 2. When the density reaches 4 x 10(4) cells/well, the OD value increased gradually and reached a peak on Day 5. After that, the OD value began to fall. The growth of IEC-6 cells was limited at a low density (0.2 x 10(4) cells/well) on Day 4. DFMO caused a complete inhibition of proliferation of IEC-6 cells on Days 1 to 3.
CONCLUSIONProliferation of IEC-6 cells is related to plating density and incubation time. It is inhibited by DFMO, but is reversible when the incubation time is prolonged.
