Comparative study of catheter-mediated gene transfer into heart.
- Author:
Jianjun LI
1
;
Gengshan LI
;
Congxin HUANG
;
Hong JIANG
;
Qizhu TANG
;
Jiali XU
;
Hao XIA
;
Jing WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Cardiac Catheterization; Dogs; Female; Gene Expression; Gene Transfer Techniques; Genetic Vectors; administration & dosage; genetics; Inflammation; etiology; Injections; adverse effects; methods; Lac Operon; genetics; Liver; metabolism; Male; Myocardium; metabolism
- From: Chinese Medical Journal 2002;115(4):612-613
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI).
METHODSFifteen dogs were used, and 0.3 ml (1 x 10(9) pfu) of an adenovirus (Adex1SR LacZ) was injected into the heart by 3 methods. The dogs were killed 5 days following injection, and gene expressions in heart and liver were evaluated by histochemical analysis.
RESULTSThe results showed that (1) the CAP method was relatively less damaging and induced sparse LacZ expression in the myocardium, and the gene expression was also found in both vessels within the myocardium and liver; (2) gene transfer by DMI resulted in intense LacZ expression around the injection accompanied by a local inflammatory response; (3) LacZ expression elicited by ICI was detected in either the inner surface of the parietal pericardium or epicardial surface of the heart, and also in the myocardium underlying the visceral pericardium.
CONCLUSIONThree catheter-mediated methods of gene transfer into the heart may be used and a reasonable approach should be chosen according to purpose.
