Nitric oxide synthase gene expression in injured spinal cord tissue.
- Author:
Chenglong LIU
1
;
Anmin JIN
;
Chusong ZHOU
;
Bin CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Gene Expression Regulation, Enzymologic; Male; Nitric Oxide Synthase; genetics; Nitric Oxide Synthase Type I; Nitric Oxide Synthase Type II; Nitric Oxide Synthase Type III; RNA; genetics; metabolism; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Spinal Cord; enzymology; pathology; Spinal Cord Injuries; enzymology; genetics
- From: Chinese Medical Journal 2002;115(5):740-742
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate gene expression of three nitric oxide synthase isozymes in injured spinal cord tissue.
METHODSThirty-six adult SD rats were randomly divided into six groups: a normal group and five injury groups, with six per each group. Animals in the injury groups were sacrificed at 2, 6, 12, 24, 48 h after injury. A compression injury model on the spinal cord was made according to Nystrom B et al and gene expression of the three NOS isozymes were examined by reverse transcription polymerase chain reaction (RT-PCR).
RESULTSGene expression of nNOS and eNOS were detectable in the normal group and were up-regulated quickly after injury, reaching a maximum at 6 h: (0.633 +/- 0.012) and (1.236 +/- 0.207). Gene expression of iNOS was detectable only in the injury groups and it was gradually up-regulated after injury, reaching a maximum at 24 h: (1.043 +/- 0.049).
CONCLUSIONInjury to the spinal cord leads to early up-regulation of cNOS and late up-regulation of iNOS. Different NOS isozymes may play different roles in secondary spinal cord injury.
