Prognostic value of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in bladder carcinoma.
- Author:
Kexin XU
1
;
Shukun HOU
;
Zhijun DU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Matrix Metalloproteinase 2; genetics; Middle Aged; Neoplasm Staging; Predictive Value of Tests; Prognosis; RNA, Messenger; genetics; metabolism; Tissue Inhibitor of Metalloproteinase-2; genetics; Urinary Bladder; metabolism; pathology; Urinary Bladder Neoplasms; genetics; pathology
- From: Chinese Medical Journal 2002;115(5):743-745
- CountryChina
- Language:English
-
Abstract:
OBJECTIVESTo detect the level of matrix metalloproteinase-2 (MMP-2) mRNA and the tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA in bladder transitional cell carcinoma (BTCC), and to estimate the prognosis for bladder tumor based on the quality and quantity of MMP-2 and TIMP-2 mRNA.
METHODSThirty-five samples of human BTCC and 15 normal fresh bladder tissues were studied by RT-PCR analysis followed by computer-assisted image analysis.
RESULTSThe level of the MMP-2 mRNA in BTCC was significantly increased compared with that in normal bladder epithelium. The positive rates of MMP-2 and TIMP-2 mRNA were 71.4% and 65.7% in BTCC, and 66.7% and 60.0% in the normal bladder wall. The expression intensity of the MMP-2 mRNA by image analysis tended to increase with tumor grading and staging, which showed statistical significance. Similarly, the MMP-2 to TIMP-2 ratio also showed statistically significant difference between normal bladder tissue and bladder carcinoma (P < 0.01).
CONCLUSIONSA high level of the MMP-2 mRNA exists in BTCC, which may function to damage collagen IV inside the basement membrane and the extracellular basement of the bladder. The level of the MMP-2 mRNA is proportional to BTCC grading and staging, which may have prognostic value. The MMP-2 to TIMP-2 ratio may play a more significant role in the determination of the aggressiveness and prognosis of bladder tumor.
