Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies.
- Author:
Qiang ZHANG
1
;
Lin CHEN
;
Lili LIANG
;
Zhijun XI
;
Yi DING
;
Ming TONG
;
Zhiwen ZHANG
;
Chung LEE
;
Yinlu GUO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; genetics; physiology; Carcinoma, Renal Cell; genetics; pathology; Cell Division; genetics; physiology; Cell Line; Cloning, Molecular; DNA, Antisense; genetics; physiology; DNA, Complementary; chemistry; genetics; Gene Expression Regulation, Neoplastic; Genes, Neoplasm; genetics; Humans; Kidney Neoplasms; genetics; pathology; Neoplasm Proteins; genetics; Oligonucleotides; genetics; Sequence Analysis, DNA; Transfection; Tumor Cells, Cultured
- From: Chinese Medical Journal 2002;115(5):746-749
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo clone the full length of renal cell carcinoma (RCC) related novel gene GYLZ-RCC18 and study its function.
METHODSSMART RACE technology was used to clone the full length of GYLZ-RCC18. RT-PCR was used to detect its expression in renal cell carcinoma tissue at different stages and grades. We transfected the antisense oligonucleotide of GYLZ-RCC18 to renal cell carcinoma cell line, GRC-1, and analyzed proliferation activity, growth rate, apoptosis, and mortality changes.
RESULTSThe full length of GYLZ-RCC18 (GenBank accession number: BE825133) cDNA was about 3.5 kb. GYLZ-RCC18 had a higher expression in higher grades and stages of renal cell carcinoma than in lower ones. The expression of GYLZ-RCC18 in renal cell carcinoma was much higher than in normal kidney. After the transfection of GYLZ-RCC18 antisense oligonucleotide, the mortality of GRC-1 increased significantly, while proliferative activity and growth rate were substantially inhibited at the same time. The antisense oligonucleotide induced apoptosis of GRC-1 through the entire observation time.
CONCLUSIONGYLZ-RCC18 is an important novel gene related to renal cell carcinoma. Overexpression of this gene results in higher growth and proliferative activity and has an antiapoptosis effect on renal cell carcinoma cells. Transfection of the antisense oligonucleotide may inhibit the generation and development of renal cell carcinoma.
