Cytogenetic analysis of 362 cases of chronic myeloid leukemia.
- Author:
Hai-Rong QIU
1
;
Kou-Rong MIAO
;
Si-Xuan QIAN
;
Rong WANG
;
Ming HONG
;
Chun QIAO
;
Jian-Fu ZHANG
;
Lei FAN
;
Han-Xin WU
;
Hua LU
;
Hong-Xia QIU
;
Li-Juan CHEN
;
Peng LIU
;
Su-Jiang ZHANG
;
Wei XU
;
Jian-Yong LI
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province People Hospital, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Child;
Female;
Humans;
Karyotyping;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
genetics;
Male;
Middle Aged;
Philadelphia Chromosome;
Young Adult
- From:
Journal of Experimental Hematology
2009;17(1):27-30
- CountryChina
- Language:Chinese
-
Abstract:
In order to evaluate the cytogenetic features and clinical significance of chronic myeloid leukemia (CML), chromosome preparation of bone marrow cells was made by using 24-hour culture, and R-banding technique was employed for karyotyping in 362 patients with CML. The patients were divided into two groups of chronic phase (CP) and blast crisis (BC). The results showed that the incidence of additional chromosome, variant translocation and Philadelphia (Ph) negative, bcr/abl positive CML with abnormal chromosomes in CP group were 70 cases (26.1%), 19 cases (7.1%), 4 cases (1.5%), and were 50 cases (53.2%), 8 cases (8.5%), 4 cases (4.3%) in BC group. Among the 362 cases, 324 cases (89.5%) were Ph positive. Classic translocation was found in 297 cases (91.7%) and variant translocation in 27 cases (8.3%), including 13 cases of simple variant, 13 cases of complex variant and 1 case of marked Ph. Special karyotypes were found in 120 out of 362 cases. Analysis of these karyotypes demonstrated that the most common numerical abnormalities were +Ph (21.7%), +8 (10.0%), +21 (10.0%), +19 (7.5%) and structure abnormalities were i(17q) (13.3%). In conclusion, compared to chronic phase, the incidence of additional chromosome, variant translocation and so on are much higher at in blast crisis. It is feasible to evaluate the progress of the disease by karyotype analysis.
