Effects of magnetic nanoparticle of fe(3)o(4) and 5-bromotetrandrine on apoptosis of K562/A02 leukemic cells induced by daunorubicin.
- Author:
Ming-Fang SHEN
1
;
Bao-An CHEN
;
Jian CHENG
;
Feng GAO
;
Wen-Lin XU
;
Jia-Hua DING
;
Chong GAO
;
Xin-Chen SUN
;
Guo-Hong LI
;
Wen-Ji CHEN
;
Li-Jie LIU
;
Xiao-Mao LI
;
Xue-Mei WANG
Author Information
1. Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical School, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Benzylisoquinolines;
pharmacology;
Daunorubicin;
pharmacology;
Down-Regulation;
Ferric Compounds;
administration & dosage;
Gene Expression Regulation, Leukemic;
Humans;
K562 Cells;
Nanoparticles;
administration & dosage;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Up-Regulation;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Experimental Hematology
2009;17(1):54-59
- CountryChina
- Language:English
-
Abstract:
The aim of this study was to investigate the potential benefit of combination therapy with magnetic nanoparticle of Fe(3)O(4) and 5-Bromotetrandrine (5-BrTet) on chronic leukemia. The apoptosis was detected by flow cytometry (FCM), Wright staining and light microscope; the expressions of BAX and BCL-2 were measured by Western blot. The results showed that combination of daunorubicin (DNR) with either MNP (Fe(3)O(4)) or 5-BrTet exerted a potent cytotoxic effect on K562/A02 cells, while MNP (Fe(3)O(4)) and 5-BrTet co-treatment could synergistically enhance DNR-induced apoptosis. After treated with this regimen, the typical apoptotic morphological features were found in K562/A02 cells; the expression level of BCL-2 decreased and BAX increased markedly. It is concluded that MNP (Fe(3)O(4)) or 5-BrTet with DNR can induce apoptosis in K562/A02 cells, and they show distinct synergism when used together. The down-regulation of BCL-2 and the up-regulation of BAX may play important roles.
