Mechanism of gambogic acid-induced apoptosis in Raji cells.
- Author:
Yong WANG
1
;
Yan CHEN
;
Zi CHEN
;
Wen-Juan KE
;
Qiu-Ling WU
;
Jing HE
Author Information
1. Institute of Hematology, Huazhong University of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
genetics;
metabolism;
Cell Line, Tumor;
DNA-Binding Proteins;
genetics;
metabolism;
Humans;
Xanthones;
pharmacology;
bcl-X Protein;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2009;17(1):88-91
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to explore the apoptotic effect of gambogic acid on Raji cells and the role of death inducer-obliterator 1 (DIO-1) in this process. Annexin V-fluorescein-isothiocyanate/propidium iodide was used to detect apoptosis of Raji cells. Western blot was used to determine the expressions of DIO-1, Bcl-xL, pro-caspase 3 and 2 activated subunits: P17 and P20. The subcellular localization of DIO-1 in untreated and treated Raji cells was checked by immunofluorescence and Hoechst 33258 double staining. The results showed that the Gambogic acid dose-dependently induced the apoptosis of Raji cells, downregulated the expression of Bcl-xL, upregulated the expressions of DIO-1 and pro-caspase 3, induced the cleavage of pro-caspase 3 and DIO nuclear translocation. It is concluded that gambogic acid induces the apoptosis of Raji cells through DIO-1 upregulation, nuclear translocation, Bcl-xL downregulation and caspase 3 activation.
