Inhibition effect of gambogic acid on MUTZ-1 cell line and its possible mechanism.
- Author:
Tie-Yan HONG
1
;
Bao-An CHEN
;
Chong GAO
;
Guo-Hua XIA
;
Jia-Hua DING
;
Ze-Ye SHAO
;
Yun-Yu SONG
;
Ju WANG
;
Jian CHENG
;
Hui-Hui SONG
;
Meng XUE
;
Wen BAO
;
Gang ZHAO
;
Wei XAO
;
Zhen-Zhong WANG
Author Information
1. Department of Hematology, Zhongda Hospital, Southeast University, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Line;
Flow Cytometry;
Humans;
Proto-Oncogene Proteins c-bcl-2;
genetics;
RNA, Messenger;
genetics;
Xanthones;
pharmacology;
bcl-2-Associated X Protein;
genetics
- From:
Journal of Experimental Hematology
2009;17(2):373-376
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effects of gambogic acid on the cells of high-risk patients with myelodysplastic syndrome (MDS) in vitro and its mechanism. The inhibition effect of gambogic acid on growth of MUTZ-1 cell line of MDS-RAEB was detected by MTT method. Apoptosis and cell cycle were detected by morphological observation and flow cytometry respectively. The expressions of bax/bcl-2 gene at mRNA level were detected by RT-PCR. The results indicated that the Gambogic acid inhibited the growth of MUTZ-1 cells, the inhibitory rate of gambogic acid with the range of 0.2 - 0.8 microg/ml was enhanced along with increasing of drug concentration. Flow cytometric assay showed that the apoptotic rate of MUTZ-1 cells treated by gambogic acid also was enhanced along with increasing of drug concentration, the apoptotic rates resulting from gambogic acid (0, 0.4, 0.6, 0.8 microg/ml) were (5 +/- 0.5)%, (13 +/- 0.5)%, (37 +/- 0.7)% and (56 +/- 0.6)% respectively. The characteristic changes of apoptosis emerged in MUTZ-1 cells after being exposed to gambogic acid. Gambogic acid could significantly down-regulate the expressions of bcl-2 gene in a dose dependent manner, however, it had no effects on bax gene. It is concluded that within the range of concentration from 0.4 to 0. 8 microg/ml, gambogic acid can inhibit the growth of MUTZ-1 cells by inducing their apoptosis and down-regulating the expression of bcl-2 gene, which may be one of the main mechanisms underlying its antitumor effects.
