Comparison of biological characteristics of bone marrow mesenchymal stem cells in acute myeloid leukemia with those from non-leukemia.
- Author:
Wen-Jie YIN
1
,
2
;
Ping-Di YANG
;
Yu-Zhang HUANG
;
Xiao-Peng LIU
;
Li-Zhong GONG
Author Information
1. Department of Hematology, Naval Clinical Medical College, The Second Military Medical University, China
2. PLA Naval General Hospital, Beijing 100037, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
cytology;
Cell Cycle;
Cell Fusion;
Cell Proliferation;
DNA;
genetics;
Humans;
Immunophenotyping;
Leukemia, Myeloid, Acute;
genetics;
immunology;
pathology;
Mesenchymal Stromal Cells;
cytology
- From:
Journal of Experimental Hematology
2009;17(2):395-399
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to compare partial biological characteristics of bone marrow mesenchymal stem cells (BMMSCs) in AML patients, AML patients with complete remission (CR) and non-leukemia patients. The bone marrow (BM) MSCs were divided into 3 groups: group of MSCs from AML patients, group of MSCs from AML patients with CR, group of MSCs from non-leukemia patients. The morphologic features of MSCs were observed by light microscopy; CFU-F numbers of MSCs were counted after Wright-Giemsa staining in situ; the fusion times of MSCs were determined; the growth curves of MSCs were drawn by counting cell numbers; the immunophenotypes and cell cycle of MSCs were detected by flow cytometry; the DI values of MSCs were calculated. The results showed that the morphologic features of MSCs in 3 groups did not display difference; there was significant difference (p < 0.01) of CFU-F numbers in 3 groups, while CFU-F number of MSCs in AML group was minimal; there was significant difference of MSC fusion time in 3 groups, while fusion time of MSCs in AML group was most long; the growth curves of MSCs in 3 groups were similar; MSCs in 3 groups highly expressed CD105 and CD106, but not expressed CD45; the cell distribution ratios at phase of G(0)/G(1) for MSCs in 3 groups were 89.9 +/- 4.0%, 90.2 +/- 3.0% and 91.0 +/- 3.0% respectively; the DI values of MSCs in 3 groups were between 0.9 and 1.1. It is concluded that no significant difference of biological characteristics of the second generations of MSCs is found between those in leukemia and non-leukemia patients.
