Comparison of migration characteristics of MSCs in different assay systems.
- Author:
Xin-Jun WANG
1
;
Jun-Ming TANG
;
Xia KONG
;
Ling-Yun GUO
;
Jian-Ye YANG
;
Fei ZHENG
;
Long CHEN
;
Yong-Zhang HUANG
;
Jia-Ning WANG
Author Information
1. Laboratory of Function Experiment, People Hospital, Hubei Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cell Movement;
Flavonoids;
pharmacology;
Mesenchymal Stromal Cells;
cytology;
Protein Kinase C;
metabolism;
Rats;
Rats, Wistar;
Receptors, CXCR4;
metabolism;
Signal Transduction
- From:
Journal of Experimental Hematology
2009;17(2):404-407
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the difference of MSC migration mediated by SDF-1/CXCR4 axis through Boyden chamber in vitro migration assay. The SDF-1 density-dependence of MSC migration was observed. Subsequently, the effects of different blocking agents on hSDF-MSC migration were observed after MSC were treated with 50 nmol/L wortmannin, 10 micromol/L LY294002, 50 micromol/L PD98059, 10 micromol/L U73122, 126 micromol/L AMD3100 and 50 nmol/L verapamil respectively. The results showed the efficiency of MSC migration increased gradually with the increasing of hSDF-1 density. And after MSCs treatment with 50 nmol/L wortmannin, 10 micromol/L LY294002, 50 micromol/L PD98059, 10 micromol/L U73122 and 126 micromol/L AMD3100 respectively, the ability of MSC migration decreased. The ability of MSCs migration obviously decreased when MSCs were treated with U73122, AMD3100. It is concluded that the SDF-1/CXCR4-mediated MSC migration may be related to mitogen-activated protein kinase (MAPK), phosphatidylinositol phospholipase C (PI-PLC) and protein kinase (PKC) signal pathways.
