Influence of high mobility group box 1 on migration of human cord blood CD34(+) cells.
- Author:
Xin CHEN
1
;
Xing-Bing WANG
;
Hui-Lan LIU
;
Wen YAO
;
Kai-Di SONG
;
Zi-Mi SUN
Author Information
1. Department of Hematology, Anhui Medical Uiversicity, Hefei 230032, Anhui Province, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
Cell Movement;
drug effects;
Cells, Cultured;
Female;
Fetal Blood;
cytology;
drug effects;
HMGB1 Protein;
pharmacology;
Humans;
Receptor for Advanced Glycation End Products;
Receptors, Immunologic;
metabolism;
Signal Transduction
- From:
Journal of Experimental Hematology
2009;17(2):422-425
- CountryChina
- Language:Chinese
-
Abstract:
The objective of study was to explore the influence of high mobility group box 1 (HMGB1) on migration of cord blood CD34(+) cells and their mechanism of migration. The expressions of receptor for advanced glycation end products (RAGE), toll-like receptor-2 (TLR2) and TLR4 were detected by flow cytometry. The CD34(+) cells in umbilical cord blood (CB) were enriched by MiniMACS and were exposed to various concentration of HMGB1 (10, 50, 100, 1, 000 ng/ml), then the migration effect of HMGB1 on umbilical cord blood (UCB) CD34(+) cell count was determined by microscopy, the chemotactic index was calculated. The CD34(+) cells untreated with HMGB1 were used as control. The results indicated that the purity of the isolated CD34(+) cells was more than 98%. The HMGB1 could promote the migration of CD34(+) cells, and the migration effect of HMGB1 on CD34(+) cells in certain concentrations gradually increased along with raise of concentration, the strongest effect was observed in concentration of 100 ng/ml, there was significant difference as compared with control (p < 0.01). Anti-RAGE antibody partially inhibited the migration effect of HMGB1 on CD34(+) cells. It is concluded that the HMGB1 in certain concentration can enhance migration of CD34(+) cells, which may be mediated through RAGE.
