Relationship between hepatitis B virus genotype, BCP/Pre-C region mutations and risk of hepatocellular carcinoma in Guangxi Zhuang Autonomous Region.

Dan Zheng; Wei Deng; Tianren Huang; Email: Tianrenhuang@sinacom; Xiliang LI; Zhaofa LI

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Relationship between hepatitis B virus genotype, BCP/Pre-C region mutations and risk of hepatocellular carcinoma in Guangxi Zhuang Autonomous Region.

Author: Dan ZHENG ¹ ; Wei DENG ² ; Tianren HUANG ³ ; Email: TIANRENHUANG@SINA.COM. ; Xiliang LI ¹ ; Zhaofa LI ¹
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1. Guangxi Medical University, Nanning 530021, China.
2. Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
3. Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China
Publication Type:Journal Article
MeSH: Carcinoma, Hepatocellular; epidemiology; virology; Case-Control Studies; China; epidemiology; DNA, Viral; Genotype; Hepatitis B virus; genetics; Humans; Incidence; Liver Neoplasms; epidemiology; virology; Mutation; Polymerase Chain Reaction; Risk Factors; Sequence Analysis, DNA
From: Chinese Journal of Epidemiology 2015;36(7):725-729
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the relationship between hepatitis B virus (HBV) genotype, the mutation in basic core promoter (BCP) region/pre-core (Pre-C) region and the incidence of hepatocellular carcinoma (HCC) in Fusui county of Guangxi Zhuang Autonomous Region (Guangxi), a area with high incidence of HCC.
METHODSIn this case-control study, 53 HCC patients and 70 asymptomatic HBV carriers were enrolled. Blood samples were collected from them for serum separation and HBV DNA extraction. The DNA sequences of the S region and BCP/Pre-C region of HBV was determined by direct sequencing following nested-PCR amplification. The relationship between the genotype, gene mutation of HBV and the incidence of HCC was analyzed.
RESULTSThe mutation rates of the A1762T/G1764A in the BCP region and the T1858C in the Pre-C region of HBV were significantly higher in HCC group than in control group (94.3% vs. 75.7%, P = 0.006; 50.9% vs. 31.4%, P = 0.029). The mutation rate of A1775G was significantly higher in control group (28.6%) than in HCC group (13.2%) (P = 0.041). Multiple logistic regression analysis indicated that A1762T/G1764A and T1858C mutations are the risk factors for the development of HCC (OR = 5.459, 95% CI: 1.397-21.332, P = 0.015; OR = 3.881, 95% CI: 1.462-10.305, P = 0.006). A1775G is the protective factor in the development of HCC (OR = 0.192, 95% CI: 0.059-0.622, P = 0.006).
CONCLUSIONThe present investigation showed that BCP A1762T/G1764A, A1775G and Pre-C T1858C mutations are correlated with the incidence of HCC in Fusui county of Guangxi.