Expression and clinical implication of soluble resistance-associated calcium-binding protein gene and multi-drug resistance gene in leukemia.
- Author:
Guangyao LI
1
;
Yaohong TAN
;
Chunzheng YANG
;
Chunhua ZHAO
;
Hongguo ZHAO
;
Jianxiang WANG
;
Yanping XUE
;
Mingzhe HAN
;
Linsheng QIAN
;
Chunting ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; genetics; Acute Disease; Blotting, Northern; methods; Calcium-Binding Proteins; genetics; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Gene Expression; Humans; K562 Cells; Leukemia, Myeloid; genetics; physiopathology; Neoplasm Proteins; genetics; Prognosis
- From: Chinese Journal of Oncology 2002;24(4):370-374
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the relationship between soluble resistance-related calcium-binding protein (sorcin) gene and multidrug resistance gene (mdr1), and their significance in clinical drug resistance and prognosis of acute myeloid leukemia (AML).
METHODSAmplification of sorcin gene and mdr1 gene in K562/A02 cell detected by Northern blot, were monitored by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) in 65 AML patients and 27 normal controls, with their relationship and clinical outcame analyzed.
RESULTSThe amplification of sorcin gene and mdr1 gene in AML patients were significantly higher than that in the normal control, which were related to clinical drug resistance and prognosis. The amplification of sorcin gene was related to the amplification of mdr1 gene in the two groups. The clinical drug resistance incidence rate and complete remission rate were 92.9% and 7.1% in sorcin(+)/mdr1(+) group. They were 8.6% and 91.4% in the sorcin(-)/mdr1(-) group (P < 0.001).
CONCLUSIONThe co-amplification of sorcin and mdr1 gene can be taken as a good indicator of clinical drug resistance and prognosis of AML.
