Effect of human calcyclin binding protein encoding gene on development of multiple drug resistance in gastric cancer.
- Author:
Wenhua HU
1
;
Fang YIN
;
Xiaohang JIN
;
Daiming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Calcium-Binding Proteins; genetics; physiology; DNA, Complementary; analysis; Drug Resistance, Multiple; physiology; Drug Resistance, Neoplasm; physiology; Drug Screening Assays, Antitumor; Gene Transfer Techniques; Humans; Molecular Sequence Data; Plant Lectins; Stomach Neoplasms; pathology; Tumor Cells, Cultured
- From: Chinese Journal of Oncology 2002;24(5):426-429
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of human calcyclin binding protein (CacyBP) encoding gene on the development of multiple drug resistance in gastric cancer.
METHODShCacyBP sense nucleic acid eukaryotic expression vector (pcDNA3.1/hCacyBP +) was constructed and then transfected steadily into the gastric cancer drug sensitive cell (SGC7901) mediated by lipofectamine ( trade mark ) 2000. RT-PCR was used to measure the CacyBP mRNA expression level. MTT was used to measure the adriamycin (ADR) drug sensitivity of SGC7901 and SGC7901 after transfection. FCM was used to measure the average ADR accumulation concentration and cell cycle of SGC7901 and SGC7901 after transfection.
RESULTSThe hCacyBP mRNA expression level of SGC7901 transfected with pcDNA3.1/hCacyBP + was higher than SGC7901 transfected with pcDNA3.1 or SGC7901, with the higher survival rate in the former. The average ADR accumulation concentration in SGC7901 and SGC7901 transfected with pcDNA3.1 or pcDNA3.1/hCacyBP + was 5.64, 5.49 and 5.17, respectively. The G(1) phase cell proportion of SGC7901 transfected with pcDNA3.1/hCacyBP + or pcDNA3.1 was reduced slightly but G(2) and S phases increased slightly as compared with SGC7901.
CONCLUSIONCalcyclin binding protein may play a certain role in gastric cancer drug resistance.
