Allelic analysis on chromosome 5 in sporadic colorectal cancer patients.
- Author:
Fang ZHANG
1
;
Chongzhi ZHOU
;
Yun LING
;
Guoqiang QIU
;
Shaochun BAI
;
Wanqing LIU
;
Lin HE
;
Zhihai PENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Alleles; Chromosome Deletion; Chromosomes, Human, Pair 5; Colorectal Neoplasms; genetics; Female; Genes, Tumor Suppressor; Humans; Loss of Heterozygosity; Male; Microsatellite Repeats; genetics; Middle Aged
- From: Chinese Journal of Oncology 2002;24(5):458-460
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate and map the putative tumor suppressor loci on chromosome 5 involved in tumor progress or metastasis.
METHODSChromosome 5 of 83 patients with sporadic colorectal cancer was systemically screened. Fifteen microsatellite marker primers labeled with 3 different fluorescents were used to amplify the corresponding loci of the genome DNA. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed with Genotyper and Genescan software.
RESULTSThe highest loss of heterozygosity (LOH) ratio was found at D5S416 (48.15%) on 5p and at D5S471 (38.71%) on 5q. The region (5q13.3 - 31), where D5S471 and 3 neighboring loci (D5S428, D5S2027 and D5S2115) reside, presented high frequent LOH.
CONCLUSIONThe deletion of APC, MCC, CTNNA1 and IL cluster in the 5q 13.3 - 31.1 area play important role in the tumorogenesis of colorectal cancer, and the expected existence of another novel tumor suppressor gene on 5p is possible.
