cDNA array in the establishment of a gene expression profile associated with differentiation inducing the glioma cells.
- Author:
Lijun SUN
1
;
Qiang HUANG
;
Aidong WANG
;
Qing LAN
;
Ziwei DU
;
Gengxi HU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; DNA, Neoplasm; analysis; Gene Expression; Gene Expression Profiling; Glioma; genetics; metabolism; pathology; Humans; Oligonucleotide Array Sequence Analysis; RNA, Neoplasm; analysis
- From: Chinese Journal of Oncology 2002;24(3):222-225
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEEstablishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible.
METHODThe expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot.
RESULTSNinety-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also.
CONCLUSIONA gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.
